封闭接头配套试剂
产品详情
产品性能
下单流程
货号信息
资料下载
产品详情
产品介绍
在杂交捕获过程中，接头之间的退火杂交，会在不同插入片段的文库之间形成串联结构，探针在捕获目标区域片段的同时也会捕获与之串联的非靶标片段，导致On-Target低，有效数据减少。而特异性1对1 Blocker使用较为繁琐，容易出现操作失误。因此，我们为MGI和Illumina测序平台均开发了相应的Universal Blocker，可高效封闭不同Index文库，同时兼容单端和双端Index等多种文库结构。
以Universal Blockers-ILMN-TS(Du)为例说明，该产品是针对illumina Truseq接头开发的通用阻断剂，如图所示，可适用于单端、双端以及双端+UMI等Truseq接头文库。
产品优势
- 高效封闭多文库捕获中的index序列
- 降低adapters序列之间的干扰，提升on-targeting
- 兼容匹配多种类型文库
预混液，便于实验操作
产品性能
超强稳定性
使用不同批次Universal Blockers-ILMN-TS(Du)，对单端和双端index Truseq接头文库进行杂交捕获，采用友商I的通用阻断剂作为对照。三个批次的通用阻断剂数据表现稳定。
Single-Index
|
Blockers
|
Rep 1#
|
Rep 2#
|
Rep 3#
|
Rep 4#
|
|---|---|---|---|---|
|
友商 I
|
54.2%
|
57.6%
|
55.0%
|
54.8%
|
|
伯科-LOT-1#
|
54.0%
|
57.8%
|
–
|
–
|
|
伯科-LOT-2#
|
–
|
–
|
55.4%
|
55.0%
|
Dual-Index
|
Blockers
|
Rep 1#
|
Rep 2#
|
Rep 3#
|
Rep 4#
|
|---|---|---|---|---|
|
友商 I
|
55.9%
|
55.2%
|
55.7%
|
57.1%
|
|
伯科-LOT-1#
|
56.3%
|
55.1%
|
57.0%
|
55.6%
|
|
伯科-LOT-2#
|
56.8%
|
55.8%
|
55.9%
|
56.0%
|
|
伯科-LOT-3#
|
55.2%
|
56.1%
|
56.5%
|
56.2%
|
图1. 对于单端和双端index均有良好的封闭性能，捕获效率
表现与友商I相似，同时，三个批次的通用阻断剂数据表现稳定。
有效避免标签错配
多文库捕获时，阻断剂设计缺陷，合成质量低都可能造成index-hopping现象，导致样本之间的污染。使用伯科Universal Blockers-ILMN-TS(Du)和友商I产品对已知SNP的两个样本进行2-Plex杂交捕获，通过分析等位基因频率对index-hopping现象进行检测。
图2. 伯科Universal Blockers-ILMN-TS(Du)能够将index-hopping
程度控制在友商I的范围内。
用户测评
不同测序平台的用户反馈的测序数据来看，在使用伯科设计生产的UniversalBlocker后，捕获效率高于友商，且变异系数低于友商
Illumina 测评结果
|
Blockers
|
On-target(average)
|
CV
|
|---|---|---|
|
伯科
|
57.43%
|
3.78%
|
|
友商
|
56.39%
|
6.40%
|
22 Plasma/FFPE/WBC Samples
MGI 测评结果
|
Sample ID
|
友商
|
伯科
|
|---|---|---|
|
1 #
|
55.92%
|
56.69%
|
|
2 #
|
58.32%
|
59.24%
|
|
3 #
|
59.64%
|
59.24%
|
|
4 #
|
58.39%
|
59.86%
|
|
5 #
|
57.52%
|
57.24%
|
|
Average
|
57.96%
|
58.47%
|
下单流程
联系伯科
电话：0510-68108516
邮箱： info@bokebiotech.com
沟通设计方案
技术专家团队为您提供
项目咨询
生产与交付
智能工厂极速交付产品
产品多重质控
售后服务
项目管理和技术支持小组
提供售后服务
货号信息
|
类别
|
产品
|
反应数
|
货号
|
体积
|
存储温度
|
说明
|
|---|---|---|---|---|---|---|
|
封闭接头
|
TargetCap® Universal Blockers-ILMN-TS-i5
|
–
|
TC00105
|
–
|
–
|
Illumina Truseq i5端接头
|
|
TargetCap® Universal Blockers-ILMN-TS-i7
|
–
|
TC00107
|
–
|
–
|
Illumina Truseq i7端接头
|
|
|
16 rxn TargetCap® Universal Blockers-ILMN-TS(Du)
|
16 rxn
|
TC0010D1
|
–
|
–
|
–
|
|
|
96 rxn TargetCap® Universal Blockers-ILMN-TS(Du)
|
96 rxn
|
TC0010D9
|
–
|
–
|
Illumina Truseq i7端接头
|
|
|
4x 96 rxn TargetCap® Universal Blockers-ILMN-TS(Du)
|
96 rxn
|
TC0010D
|
–
|
–
|
–
|
|
|
16 rxn TargetCap® Universal Blockers-ILMN-NT
|
16 rxn
|
TC00111
|
–
|
–
|
–
|
|
|
96 rxn TargetCap® Universal Blockers-ILMN-NT
|
96 rxn
|
TC00119
|
–
|
–
|
Illumina Nextera文库接头
|
|
|
4x 96 rxn TargetCap® Universal Blockers-ILMN-NT
|
96 rxn
|
TC0011
|
–
|
–
|
–
|
|
|
16 rxn TargetCap® Universal Blockers-MGI(Du)
|
16 rxn
|
TC0012D1
|
–
|
–
|
–
|
|
|
96 rxn TargetCap® Universal Blockers-MGI(Du)
|
96 rxn
|
TC0012D9
|
–
|
–
|
MGI文库双端接头
|
|
|
4x 96 rxn TargetCap® Universal Blockers-MGI(Du)
|
96 rxn
|
TC0012D
|
–
|
–
|
–
|
|
|
16 rxn TargetCap® Universal Blockers-MGI(SI)
|
16 rxn
|
TC0012S1
|
–
|
–
|
–
|
|
|
96 rxn TargetCap® Universal Blockers-MGI(SI)
|
96 rxn
|
TC0012S9
|
–
|
–
|
MGI文库单端接头
|
|
|
4x 96 rxn TargetCap® Universal Blockers-MGI(SI)
|
96 rxn
|
TC0012S
|
–
|
–
|
–
|
|
|
96 rxn TargetCap® Universal Blockers (Customer Design)
|
96 rxn
|
TC0013
|
–
|
–
|
个性化接头
|
配套试剂组
|
类别
|
名称
|
货号
|
规格
|
试剂说明
|
组分说明
|
组分货号
|
|---|---|---|---|---|---|---|
|
illumina Truseq文库捕获试剂
|
TargetCap® Capture Kit Box 1 Illumina-TS
|
TC00311
|
96 rxn
|
探针溶解buffer
|
Probes Dissolve Buffer
|
TC0014
|
|
Blocking Reagents（ILMN-TS Blockers&Cot1）
|
Cot-1 DNA
|
TC0016
|
||||
|
96 rxn Universal Blockers-ILMN-TS(Du)
|
TC0010D9
|
|||||
|
PCR Enrichment Reagents（ILMN）
|
2x PCR ReadyMix
|
TC0018
|
||||
|
10x PCR PrimerMix(Illumina)
|
TC00191
|
|||||
|
Elution Buffer
|
TC0015
|
|||||
|
Hybridization and Wash Reagents
|
2x Hybridization Buffer
|
TC0001
|
||||
|
Hybridization Enhancer
|
TC0002
|
|||||
|
2x Beads Wash Buffer
|
TC0003
|
|||||
|
10x Wash Buffer S
|
TC0004
|
|||||
|
10x Wash Buffer I
|
TC0005
|
|||||
|
10x Wash Buffer II
|
TC0006
|
|||||
|
10x Wash Buffer III
|
TC0007
|
|||||
|
illumina Nextera文库捕获试剂
|
TargetCap® Capture Kit Box 1 Illumina-NT
|
TC00312
|
96 rxn
|
探针溶解buffer
|
Probes Dissolve Buffer
|
TC0014
|
|
Blocking Reagents（ILMN-NT Blockers&Cot1）
|
Cot-1 DNA
|
TC0016
|
||||
|
96 rxn Universal Blockers-ILMN-NT
|
TC00119
|
|||||
|
PCR Enrichment Reagents（ILMN）
|
2x PCR ReadyMix
|
TC0018
|
||||
|
10x PCR PrimerMix(Illumina)
|
TC00191
|
|||||
|
Elution Buffer
|
TC0015
|
|||||
|
Hybridization and Wash Reagents
|
2x Hybridization Buffer
|
TC0001
|
||||
|
Hybridization Enhancer
|
TC0002
|
|||||
|
2x Beads Wash Buffer
|
TC0003
|
|||||
|
10x Wash Buffer S
|
TC0004
|
|||||
|
10x Wash Buffer I
|
TC0005
|
|||||
|
10x Wash Buffer II
|
TC0006
|
|||||
|
10x Wash Buffer III
|
TC0007
|
|||||
|
MGI单端文库捕获试剂
|
TargetCap® Capture Kit Box 1 MGI-SI
|
TC00313
|
96 rxn
|
探针溶解buffer
|
Probes Dissolve Buffer
|
TC0014
|
|
Blocking Reagents（MGI-SI Blockers&Cot1）
|
Cot-1 DNA
|
TC0016
|
||||
|
96 rxn Universal Blockers-MGI(SI)
|
TC0012S9
|
|||||
|
PCR Enrichment Kit（MGI-SI）
|
2x PCR ReadyMix
|
TC0018
|
||||
|
10x PCR PrimerMix(MGI-SI)
|
TC00192
|
|||||
|
Elution Buffer
|
TC0015
|
|||||
|
Hybridization and Wash Reagents
|
2x Hybridization Buffer
|
TC0001
|
||||
|
Hybridization Enhancer
|
TC0002
|
|||||
|
2x Beads Wash Buffer
|
TC0003
|
|||||
|
10x Wash Buffer S
|
TC0004
|
|||||
|
10x Wash Buffer I
|
TC0005
|
|||||
|
10x Wash Buffer II
|
TC0006
|
|||||
|
10x Wash Buffer III
|
TC0007
|
|||||
|
MGI双端文库捕获试剂
|
TargetCap® Capture Kit Box 1 MGI-Du
|
TC00314
|
96 rxn
|
探针溶解buffer
|
Probes Dissolve Buffer
|
TC0014
|
|
Blocking Reagents（MGI-Du Blockers&Cot1）
|
Cot-1 DNA
|
TC0016
|
||||
|
96 rxn Universal Blockers-MGI(Du)
|
TC0012D9
|
|||||
|
PCR Enrichment Kit（MGI-Du）
|
2x PCR ReadyMix
|
TC0018
|
||||
|
10x PCR PrimerMix(MGI-Du)
|
TC00193
|
|||||
|
Elution Buffer
|
TC0015
|
|||||
|
Hybridization and Wash Reagents
|
2x Hybridization Buffer
|
TC0001
|
||||
|
Hybridization Enhancer
|
TC0002
|
|||||
|
2x Beads Wash Buffer
|
TC0003
|
|||||
|
10x Wash Buffer S
|
TC0004
|
|||||
|
10x Wash Buffer I
|
TC0005
|
|||||
|
10x Wash Buffer II
|
TC0006
|
|||||
|
10x Wash Buffer III
|
TC0007
|
|||||
|
链霉亲和素磁珠与文库纯化磁珠
|
TargetCap® Capture Kit Box 2 (M270 Streptavadin Beads&Purification Beads)
|
TC00321
|
96 rxn
|
含有Capture磁珠和文库纯化磁珠**
|
M270 Streptavadin Beads
|
TC0017C
|
|
Purification Beads
|
TC0017P
|
资料下载